%0 Journal Article
%T “绿岭”核桃种胚组培苗腋芽快繁体系的初步研究<br>
Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings
%A 刘喜星
%A 顾玉红
%A 李保国
%A 齐国辉
%A 刘璐
%A 回彦哲
%A 董丽欣
%A 马丽媛
%J Hans Journal of Agricultural Sciences
%P 25-29
%@ 2164-5523
%D 2012
%I Hans Publishing
%R 10.12677/hjas.2012.22005
%X <p>
	<span style=\"font-family:宋体;font-size:10pt;\">以早实薄皮核桃</span><span style=\"font-family:宋体;font-size:10pt;\">“</span><span style=\"font-family:宋体;font-size:10pt;\">绿岭</span><span style=\"font-family:宋体;font-size:10pt;\">”</span><span style=\"font-family:宋体;font-size:10pt;\">成熟种胚诱导出的腋芽为外植体，</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">MS</span><span style=\"font-family:宋体;font-size:10pt;\">为基本培养基，通过调节激素种类和浓度配比，初步研究了</span><span style=\"font-family:宋体;font-size:10pt;\">“绿岭”</span><span style=\"font-family:宋体;font-size:10pt;\">核桃种胚组培苗腋芽快繁体系。比较试验结果表明：最佳种胚萌发培养基为</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">MS + </span><span style=\"font-family:宋体;font-size:10pt;\">蔗糖</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">30 g/L + </span><span style=\"font-family:宋体;font-size:10pt;\">琼脂</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">6 g/L</span><span style=\"font-family:宋体;font-size:10pt;\">；最佳腋芽诱导和增殖培养基为</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + </span><span style=\"font-family:宋体;font-size:10pt;\">蔗糖</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">30 g/L + </span><span style=\"font-family:宋体;font-size:10pt;\">琼脂</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">6 g/L</span><span style=\"font-family:宋体;font-size:10pt;\">。生根采用两步生根法：先在</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">DKW + IBA 8.0 mg/L + </span><span style=\"font-family:宋体;font-size:10pt;\">蔗糖</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">30 g/L + </span><span style=\"font-family:宋体;font-size:10pt;\">琼脂</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">6.0 g/L</span><span style=\"font-family:宋体;font-size:10pt;\">暗培养</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">20 d</span><span style=\"font-family:宋体;font-size:10pt;\">，然后转入</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">DKW + </span><span style=\"font-family:宋体;font-size:10pt;\">蔗糖</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">30 g/L + </span><span style=\"font-family:宋体;font-size:10pt;\">琼脂</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">6 g/L</span><span style=\"font-family:宋体;font-size:10pt;\">培养基中进行光照培养，生根率为</span><span style=\"font-family:'Times New Roman','serif';font-size:10pt;\">50%</span><span
%K 核桃；核桃属；胚；腋芽；激素<br>
Walnut
%K Juglans
%K Embryo
%K Axillary Buds
%K Hormones
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=696