%0 Journal Article
%T 泥螺多肽抗肿瘤活性的初步研究<br>Anti-Tumor Activity of the Peptide from <i>Bullacta exarata</i>
%A 林焕乐
%A 励芝伊
%A 陈博
%A 易忠达
%A 王泽峰
%A 马剑茵
%J Hans Journal of Biomedicine
%P 19-22
%@ 2161-8984
%D 2012
%I Hans Publishing
%R 10.12677/HJBM.2012.23005
%X <div style=\"text-align:justify;\">
	<span style=\"line-height:1.5;\">目的</span><span style=\"line-height:1.5;\">：</span><span style=\"line-height:1.5;\">提取分离泥螺软体组织，探索其体外抗肿瘤活性。</span><span style=\"line-height:1.5;\">方法</span><span style=\"line-height:1.5;\">：</span><span style=\"line-height:1.5;\">采用胰蛋白酶酶解法获得泥螺多肽粗提物，通过超滤膜滤过技术获得分子量在</span><span style=\"line-height:1.5;\">5~10 kDa</span><span style=\"line-height:1.5;\">的组分。用葡聚糖凝胶</span><span style=\"line-height:1.5;\">G-75</span><span style=\"line-height:1.5;\">分离、纯化，</span><span style=\"line-height:1.5;\">MTT</span><span style=\"line-height:1.5;\">法测定其体外抗肿瘤活性并用荧光染色法观察细胞形态。</span><span style=\"line-height:1.5;\">结果</span><span style=\"line-height:1.5;\">：</span><span style=\"line-height:1.5;\">经膜分离得到的组分</span><span style=\"line-height:1.5;\">(</span><span style=\"line-height:1.5;\">分子量</span><span style=\"line-height:1.5;\">5~10 KD</span><span style=\"line-height:1.5;\">)</span><span style=\"line-height:1.5;\">和经葡聚糖凝胶</span><span style=\"line-height:1.5;\">G-75</span><span style=\"line-height:1.5;\">分离得到的泥螺多肽对前列腺</span><span style=\"line-height:1.5;\">PC-3</span><span style=\"line-height:1.5;\">肿瘤细胞均有一定的抑制作用，且后者强于前者。</span><span style=\"line-height:1.5;\">结论</span><span style=\"line-height:1.5;\">：</span><span style=\"line-height:1.5;\">泥螺多肽具有一定的抗前列腺</span><span style=\"line-height:1.5;\">PC-3</span><span style=\"line-height:1.5;\">肿瘤细胞的活性。&lt;br/&gt;</span><span style=\"line-height:1.5;\">Objective:</span><span style=\"line-height:1.5;\"> To isolate and purify the peptide from </span><span style=\"line-height:1.5;\">Bullacta exarata</span><span style=\"line-height:1.5;\">, and study its anti-tumor effect on prostate cancer. </span><span style=\"line-height:1.5;\">Methods:</span><span style=\"line-height:1.5;\"> The antimicrobial peptides were hydrolyzed from soft tissues of </span><span style=\"line-height:1.5;\">Bullacta exarata</span><span style=\"line-height:1.5;\"> by trypsin, and purified by sephadex chromatography. Measured by MTT assay in vitro anti-tumor activity and cell morphology was observed by fluorescence staining.</span><span style=\"line-height:1.5;\"> Results:</span><span style=\"line-height:1.5;\"> The components separated from membrane (molecular weight 5 - 10 KD)and isolated by the Sephadex G-75 could inhibitse the PC-3 cells growth, and the latter stronger than the former.</span><span style=\"line-height:1.5;\"> </span><span style=\"line-height:1.5;\">Conclusion: </span><span style=\"line-height:1.5;\">The </span><span style=\"line-height:1.5;\">Bullacta</span><span
%K 泥螺多肽；抗肿瘤；酶解；前列腺癌<br>Peptides of Bullacta Exarata
%K Anti-Tumor
%K Enzymatic
%K Prostate Cancer
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=804