%0 Journal Article
%T MASS PRODUCTION OF THE BENEFICIAL NEMATODE STEINERNEMA CARPOCAPSAE UTILIZING A FED-BATCH CULTURING PROCESS
%A Devang Upadhyay
%A Rinu Kooliyottil
%A Sivanadane Mandjiny
%A Floyd L. Inman III
%J ESci Journal of Plant Pathology
%D 2013
%I ESci Journals Publishing
%X The present study deals with the batch and fed-batch mass production of Steinernema carpocapsae. S. carpocapsae is an entomoparasitic nematode that is used as a biological control agent of soil-borne crop insect pests. The ability and efficiency of fed-batch culture process was successful through the utilization of the nematode¡¯s bacterial symbiont Xenorhabdus nematophila. Results from the fed-batch process were compared to those obtain from the standard batch process. The fed-batch process successively improved the mass production process of S. carpocapsae employing liquid medium technology. Within the first week of the fed-batch process (day six), the nematode density obtained was 202,000 nematodes mL 1; whereas on day six, batch culture mode resulted in a nematode density of 23,000 nematodes mL 1. The fed-batch process was superior to that of batch production with a yield approximately 8.8-fold higher. In fed-batch process, the nematode yield was improved 88.6 % higher within a short amount of time compared to the batch process. Fed-batch seems to make the process more efficient and possibly economically viable.
%K Steinernema carpocapsae
%K Xenorhabdus nematophila
%K fed-batch fermentation
%K beneficial nematodes
%U http://escijournals.net/EJPP/article/view/76