%0 Journal Article
%T Effect of deforolimus and VEGF on angiogenesis in endometrial stromal cells following three-dimensional culture
%A Jafar Ai
%A Somayeh Ebrahimi
%A Armin Ai
%A Roya Karimi
%A Naghmeh Bahrami
%J Stem Cell Discovery
%P 7-12
%@ 2161-6787
%D 2013
%I Scientific Research Publishing
%R 10.4236/scd.2013.31002
%X <p align=\"justify\">
	<span style=\"font-family:'Arial','sans-serif';font-size:10pt;\">The presence of endometrial tissue outside of the uterine cavity is named endometriosis and is the most common gynecologic disorder in women. Determining the inhibitory effect of a Deforolimus on angiogenesis in a three-dimensional (3-D) culture of human endometrial stromal cells (hEnCs) <i>in vitro</i>. The important mechanism in the pathogenesis of endometriosis is angiogenesis, and deforolimus has been shown to have anti-angiogenic activity. This was an <i>in vitro</i> study of human endometrial stromal cells in 3-D culture of fibrin matrix. Endometrial stromal cells isolated and placed in a 3-D fibrin matrix culture system for angiogenesis with VEGF and inhibit angiogenesis by deforolimus. Finally these cells analyzed by CD31 antibodies. After 3 weeks, in cells treated with VEGF, endothelial cell branching was observed and rudimentary capillary-like structures formed. In the presence of 5¦ÌM of deforolimus, angiogenesis was reduced. The deforolimus were shown to be effective in inhibiting the mechanisms of angiogenesis.</span> 
</p>
%K Endometrial Stromal Cells
%K Endometrium
%K 3-D culture
%K Deforolimus
%K VEGF
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=26517