%0 Journal Article
%T Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica
%A I-Ching Kuan
%A Chia-Chi Lee
%A Bing-Hong Tsai
%A Shiow-Ling Lee
%A Wei-Ting Lee
%A Chi-Yang Yu
%J Energies
%D 2013
%I MDPI AG
%R 10.3390/en6042052
%X We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 ˇăC, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 ˇăC, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 ˇăC or room temperature.
%K biodiesel
%K lipase
%K biomimetic silica
%K response surface methodology
%U http://www.mdpi.com/1996-1073/6/4/2052