%0 Journal Article
%T Ex-vivo assessment of meningococcal resistance to natural immunity: serum bactericidal assays versus whole- blood assay
%A Zahra Eslami Nejad
%A Nikoo Nik Nafs
%A Nosratollah Saeed Adeli
%J Journal of Mazandaran University of Medical Sciences
%D 2009
%I Mazandaran University of Medical Sciences
%X 1, 2,1 2 (Received 4 Dec, 2008; Accepted 14 Feb, 2009)AbstractBackground and purpose: Literature review indicates that antibiotic resistance of bacteria may be associated with the resistance to host natural immunity. In the previous study we found that: all meningococcal isolates of meningitis cases are less sensitive to typical anti-meningococcal antibiotics. In present study, the association of the above characters was analyzed in an ex-vivo ¡°case-control¡± study.Materials and methods: A randomized controlled trial compared effect two dietary treatments (low glycemic index & low fat) in 46 adults, ages 18-55 years old, BMI >27,who proceed to motahary clinic in shiraz, Iran. Body weight, BMI, WHR (waist/hip ratio), fast and post-prandial Plasma lipid profile (Triacylglycerol, total Cholesterol, LDL-C, HDL-C concentration) level of obese women measured at the beginning and at the end 6 weeks.Results: The resistance of both groups against selected sera were similar in the ratio 1:2- by SIC and SBC methods. The growth of ¡°test¡± and ¡°control¡± groups in the pooled sera were stopped at the ratios 1:512 and 1:1024 respectively. In SBA the population of ¡°test¡± and ¡°control¡± groups decreased in parallel to each other. However, the invasive (test) meningococcal strains established slower reduction in WBA.Conclusion: Studies on the correlation between antibiotic resistance of bacteria and the resistance to the natural immunity have ended with different results. According to our experience, it seems that for ex- vivo investigation of natural immunity against meningococci the WBA is more reliable. J Mazand Univ Med Sci 2009; 19(68):48-57 (Persian)
%K Neisseria meningitidis
%K meningococci
%K SIC
%K SBC
%K SBA
%K WBA
%U http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=707