%0 Journal Article
%T Evaluation of Myc Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic in-Situ Hybridization (Dual CISH) Assay
%A Daniel Lerda
%A Marta Cabrera
%A Jorge Flores
%A Luis Gutierrez
%J Journal of Interdisciplinary Histopathology
%D 2013
%I GESDAV
%R 10.5455/jihp.20130117075557
%X Objetive: The overall purpose of the study was to demonstrate applicability of the Dako dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to fluorescence in situ hybridization (FISH) probes MYC-C. Methods: MYC gene amplification by FISH and Dako dual-color CISH Results: The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual ¨Ccolor CISH assay was performed on 40 cases of prostate cancer. Amplification was identified in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. FISH data were available in total of 40 tumors. All tumors showed concordant results between dual-color CISH and FISH for classifying a tumor as MYC amplified or not amplified. Conclusions: We conclude that dual-color Dako CISH assay is an accurate method for determining MYC gene amplification with added advantages that make it a more practically useful method. [J Interdiscipl Histopathol 2013; 1(2.000): 81-84]
%K MYC
%K prostate cancer
%K chromogenic in situ hybridization
%K CISH
%U http://www.scopemed.org/fulltextpdf.php?mno=28756