%0 Journal Article
%T The epitope of the VP1 protein of porcine parvovirus
%A Hong-ling Xie
%A Zhao Wang
%A Shang-jin Cui
%A Chao-fan Zhang
%A Yu-dong Cui
%J Virology Journal
%D 2010
%I BioMed Central
%R 10.1186/1743-422x-7-161
%X Porcine parvovirus (PPV), which was first isolated from sows in Germany by Mayr et al. [1] and which belongs to the genus Parvovirus in the family Parvoviridae, is the major causative virus in a syndrome of reproductive failure in swine. This syndrome is referred to as SEDI and includes stillbirths, mummified fetuses, early embryonic death, and infertility [2]. Recent studies indicate that, in addition to inducing reproductive failure, PPV also causes dermatitis, diarrhea, and respiratory system disease [3-10].PPV is composed of structural protein and non-structural protein, and the structural protein is the virus's main immunological antigen. Using SDS-PAGE, Moliter et al. [11] identified three kinds of structural proteins: VP1, VP2, and VP3. All three of these proteins can induce hemagglutination inhibition (HI) antibodies and neutralization antibodies in rabbits; induction of HI antibodies was greatest with VP3, intermediate with VP2, and least with VP1.VP1 has an important biological function for PPV: its intranuclear signal sequence, which is similar to that of the VP1 proteins of Simian virus 40 and human papillomavirus [12], is very important for PPV positioning within the host nucleus. The N-terminal of VP1 is rich in alkaline amino acids, which enhance binding with host DNA to stabilize viral single-strand genome DNA; the binding is required for the initiation of viral DNA reproduction and for the packaging of the viral genome [13].The structural proteins of a related virus, canine parvovirus (CPV), have three antigen epitope regions In the case of CPV, the epitope located in region B1 possesses good antigenicity and induces the host to produce neutralization antibody [14,15]. Unfortunately, such information about antigen epitope is unavailable for PPV.Phage display is a powerful tool for the study of the interaction between antigen and antibody. This molecular biology technique can directly select simulated antigen epitopes that can combine to antibody in
%U http://www.virologyj.com/content/7/1/161