%0 Journal Article
%T Mediterranean river buffalo oxytocin-neurophysin I (OXT) gene: structure, promoter analysis and allele detection
%A G. Cosenza
%A A. Pauciullo
%A A. Mancusi
%A D. Nicodemo
%J Italian Journal of Animal Science
%D 2010
%I PAGEPress Publications
%R 10.4081/ijas.2007.s2.303
%X Oxytocin (OXT) is a very abundant nonapeptide neurohypophysial hormone implicated in several aspects of reproduction, including social, sexual and maternal behaviour, induction of labour and milk ejection. The nucleotide sequence of the whole OXTneurophysin I encoding gene (OXT) in Mediterranean river buffalo was determined, plus 993 nucleotides at the 5¡¯ flanking region. Buffalo oxytocin gene sequence analysis showed two transitions in the promoter region (C¡úT in position ¨C 966 and G¡úA in position ¨C 790) and one transversion G¡úT at the 170th nucleotide of the second exon, responsible for the Arg97¡úLeu aa substitution which identifies an allele named OXT B. A PCR-RFLP based method for a rapid identification of carriers of these alleles has been developed.
%K River buffalo
%K Oxytocin-neurophysin I
%K OXT
%K Nucleotide sequence
%K Allele
%K Milk ejection.
%U http://www.aspajournal.it/index.php/ijas/article/view/977