%0 Journal Article
%T Isolation and identification of a bovine viral diarrhea virus from sika deer in china
%A Yugang Gao
%A Shijie Wang
%A Rui Du
%A Quankai Wang
%A Changjiang Sun
%A Nan Wang
%A Pengju Zhang
%A Lianxue Zhang
%J Virology Journal
%D 2011
%I BioMed Central
%R 10.1186/1743-422x-8-83
%X we isolated a suspected BVDV strain from livers of an aborted fetus from sika deer in Changchun (China) using MDBK cell lines, named as CCSYD strain, and identified it by cytopathic effect (CPE), indirect immunoperoxidase test (IPX) and electron microscopy(EM). The results indicated that this virus was BVDV by a series of identification. The structural proteins E0 gene was cloned and sequenced. The obtained E0 gene sequence has been submitted to GenBank with the accession number: FJ555203. Alignment with other 9 strains of BVDV, 7 strains of classical swine fever virus (CSFV) and 3 strains of border disease virus(BDV) in the world, showed that the homology were 98.6%-84.8%, 76.0%-74.7%, 76.6%-77.0% for nucleotide sequence, respectively. The phylogenetic analysis indicated that new isolation and identification CCSYD strain belonged to BVDV1b.To the best of our knowledge, this is the first report that BVDV was isolated and identified in sika deer. This current research contributes development new BVDV vaccine to prevent and control of BVD in sika deer.Bovine viral diarrhea virus, a single-stranded RNA is found in cattle and other ruminants worldwide [1-4]. The presence of BVDV in other domestic species such as sheep or wild species such as whitetail deer might be relevant to the epidemiology other disease in cattle [5]. The BVDV infections range from clinically in apparent infections to severe disease involving one or more organ systems. Historically, BVDV was associated with digestive tract disease including high mortality. Currently, BVDV is associated more frequently with respiratory disease and fetal infections [2]. Raise skia deer already had hundreds years at present artificially in china and farmed populations had reached hundreds of thousands in recent year. However, bovine viral diarrhea (BVD) caused significantly losses in the deer population. It was reported that infections rates of BVDV for young deer reached 60%~86.7% in some areas of china in recently ye
%U http://www.virologyj.com/content/8/1/83