%0 Journal Article
%T Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china
%A Fengxue Wang
%A Xijun Yan
%A Xiuli Chai
%A Hailing Zhang
%A Jianjun Zhao
%A Yongjun Wen
%A Wei Wu
%J Virology Journal
%D 2011
%I BioMed Central
%R 10.1186/1743-422x-8-85
%X Canine distemper virus (CDV) is an enveloped negative-strand RNA virus classified into the genus Morbillivirus within the family Paramyxoviridae. CDV has a very broad host range, having been isolated from dogs, red pandas, foxes, raccoon dogs and tigers, and also identified in giant pandas, lions, lynxes, golden cats, bears and wolves [1-4]. Since 1972, the number of clinical cases of distemper in dogs and fur animals increased rapidly, when large mink and fox farms were established in China. CDV infection results in systemic disease with involvement of the central nervous system and the respiratory and gastrointestinal tracts [5]. The economic loss is significant due to CDV infection.Although vaccination against CDV with attenuated virus can protect the majority of animals, this protection does not necessarily extend to the field strains. The accurate diagnosis of CDV infection can be made with the use of methods based on molecular biological techniques. In recent years, the application of nucleic acid hybridization, PCR and other techniques[6-9] has improved the accuracy, sensitivity and specificity of CDV diagnosis. The detection of CDV based on N gene has been established by many researchers [10-14]. Recently, real-time RT-PCR targeting the hypervariable C-terminal domain of the nucleocapsid (N) gene was established and shown to be more sensitive and effective [15]. The use of RT-PCR-RFLP to detect the hemagglutinin (H) gene of CDV was reported by Calderon [16]. However, the CDV N gene is more conserved than H gene, and therefore, provides a better target for CDV detection.Identification of vaccine and field strains of CDV is very important for control the disease. The contagious nature and the high mortality rates of canine distemper make it necessary to speed up the diagnostic procedure in order to quarantine infected animals and start appropriate treatments early. CDV is a highly effective prophylactic. Accurate vaccination and good surveillance programs are
%U http://www.virologyj.com/content/8/1/85