%0 Journal Article
%T Molecular Typing of Salmonella paratyphi B and Salmonella paratyphi C Isolates from Clinical Samples in Iran
%A Fahimeh Baghbani-arani
%A Mercedeh Tajbakhsh
%A Atiyeh Hashemi Soltaniyeh
%A Bahareh Rajaei
%J Journal of Fasa University of Medical Sciences
%D 2012
%I Fasa University of Medical Sciences
%X Background & Objective: Molecular typing is an important tool in surveillance and outbreak investigations of human Salmonella infections. In this study, Subtyping of Salmonella Paratyphi B and C isolates derived from Iranian patients was carried out by RAPD-PCR to assess the extent of genetic diversity of these isolates. Materials & Methods: Fourteen Salmonella isolates including 6 strains of Salmonella paratyphi B and 8 strains of Salmonella paratyphi C were characterized using RAPD-PCR. Two arbitrary primers, namely OPP-16 and P1254 were used for RAPD analysis and the dendrograms were constructed with NTsys 2.0 computer software. Results: Both primers showed high discriminatory power in differentiating of the related strains of Salmonella. The dendrograms constructed based on RAPD-PCR profiles (with both primers) involving 14 salmonella strains revealed 4 distinct patterns, indicating that these isolates are genetically heterogeneous. Furthermore, a good correlation was not observed between the serotype and the molecular profiles obtained from RAPD data of the Salmonella isolates. Conclusion: The findings of the present study verify the usefulness of RAPD-PCR in characterizing and comparing strains of Salmonella Paratyphi B and C.
%K Salmonella
%K RAPD-PCR
%K NTsys
%K Genetic Diversity
%U http://journal.fums.ac.ir/browse.php?a_code=A-10-1-40&slc_lang=en&sid=1