%0 Journal Article
%T How pure are your vesicles?
%A Jason Webber
%A Aled Clayton
%J Journal of Extracellular Vesicles
%D 2013
%I Co-Action Publishing
%R 10.3402/jev.v2i0.19861
%X We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.
%K extracellular vesicles
%K nano-particle tracking
%K vesicle counting
%K sample purity
%U http://www.journalofextracellularvesicles.net/index.php/jev/article/view/19861/25423