%0 Journal Article
%T An overview on ELISA techniques for FMD
%A Li-na Ma
%A Jie Zhang
%A Hao-tai Chen
%A Jian-hua Zhou
%A Yao-zhong Ding
%A Yong-sheng Liu
%J Virology Journal
%D 2011
%I BioMed Central
%R 10.1186/1743-422x-8-419
%X More than 100 studies regarding ELISA method available for FMD diagnosis, antigenic analysis and monitor were thoroughly reviewed. We investigated previous sagacious results of these tests on their sensitivity, specificity.We found that in all ELISA formats for FMD, antibody-trapping and competitive ELISAs have high specificity and RT-PCR (oligoprobing) ELISA has extra sensitivity. A panel of monoclonal antibodies to different sites or monoclonal antibody in combination of antiserum is the most suitable combination of antibodies in ELISA for FMD. Even though from its beginning, 3ABC is proven to be best performance in many studies, no single NSP can differentiate infected from vaccinated animals with complete confidence. Meanwhile, recombinant antigens and peptide derived from FMDV NPs, and NSPs have been developed for use as an alternative to the inactivated virus antigen for security.There is a need of target protein, which accurately determines the susceptible animal status based on the simple, fast and reliable routine laboratory test. A further alternative based on virus-like particle (VLP, also called empty capsids) in combination of high throughput antibody technique (Phage antibody library/antibody microarray) may be the powerful ELISA diagnostic reagents in future.Foot and mouth disease(FMD) is a highly contagious and economically devastating disease of cloven-hoofed animals which hold a wide of the host spectrum such as cattle, pigs, sheep, goats, buffalo, deer, antelope and wild pigs and can severely constrain international trade of animals and animal products. FMD is caused by FMD virus (FMDV), a virus in the genus Aphthovirus within the family Picornaviridae [1]. The genome is over 8 kb in length and encode four structural proteins (SPs, VP1, VP2, VP3 and VP4)that form an icosahedrical capsid [2], and a total of ten mature non-structural proteins (NSPs)(L, 2A, 2B, 2C, 3A, 3B, 3C, 3D; or some complex, such as 3AB or 3ABC). Though the genome of FMDV is sm
%U http://www.virologyj.com/content/8/1/419