%0 Journal Article
%T Depletion of neutrophils in a protective model of pulmonary cryptococcosis results in increased IL-17A production by gamma/delta T cells
%A Wozniak Karen L
%A Kolls Jay K
%A Wormley Floyd L
%J BMC Immunology
%D 2012
%I BioMed Central
%R 10.1186/1471-2172-13-65
%X Protective responses in mice immunized with an interferon-gamma producing strain of Cryptococcus neoformans, H99¦Ã, are associated with IL-17A production by neutrophils. Neutrophil depletion in H99¦Ã-immunized mice did not affect pulmonary fungal burden, indicating that neutrophils are not required for clearance. However, we observed an increase in IL-17A in the lungs of neutrophil-depleted H99¦Ã infected mice, which corresponded to an increase in IL-17A+ ¦Ã¦Ä+ T cells. Moreover, we observed increased IL-17A+/ CD3+ cells and IL-17A+/¦Ã¦Ä+ cells, but decreased IL-17A+/Ly6G+ neutrophils in the lungs of IL-17 receptor (R)A deficient mice compared to wild-type mice. Increased production of IL-17A in neutropenic mice coincided with increased IL-6 and CXCL1, but not Th17 inducing cytokines TGF-¦Â, IL-21 and IL-23. Concurrent depletion of neutrophils and ¦Ã¦Ä+ T cells reduced IL-17A levels. Our results suggest that ¦Ã¦Ä+ T cells mediate significant IL-17A production in neutropenic mice during the protective response to C. neoformans infection.
%K Gamma-delta T cells
%K Cryptococcus neoformans
%K IL-17A
%K Pulmonary
%U http://www.biomedcentral.com/1471-2172/13/65