%0 Journal Article
%T First steps towards a new screening method for anabolic androgenic agents in human hair follicle
%A Martina Reiter
%A Sybille L¨ąderwald
%A Michael W. Pfaffl
%A Heinrich H.D. Meyer
%J Doping Journal
%D 2008
%I 
%X The idea of this feasibility study in hair follicle was to test a possible new way of detecting anabolic steroids, based on biomarker measurement for different anabolic agents. With specific biomarker expression patterns it should be possible to foster the existing doping analysis methods. For the experiment the hair follicle was chosen as suitable tissue.The first aim was to develop a method for RNA isolation out of plucked hair follicle samples. In a first study samples from untreated women and men were taken to find gender specific differences and in a second study also samples from weight lifters under doping conditions were compared with a control group. As biomarkers, different androgen dependent target genes were analyzed via qRT-PCR. Hair follicle cells from the root sheath could be taken by plucking. In the first study no significant gender dependent differences could be detected. In the second study a down-regulation of the glucocorticoid receptor could be calculated and fibroblast growth factor 7 was only expressed in treated samples. These results show first promising differences between treated and untreated samples.
%K anabolic androgenic agents
%K hair follicle cells
%K quantitative real-time RT-PCR
%K Exercise
%K Drugs
%K Cheating
%K Testing program
%K Fair play
%K Sports Competition
%K Designer drug
%K Anti-Doping
%K Doping
%K Medical
%K Sports Medicine
%K Olympic games
%K Olymic movement
%K IOC
%K International Olympic Committee
%K WADA
%U http://dopingjournal.org/content/5/3/dopingj052008-01.html