%0 Journal Article
%T Persistent activation of NF-kappaB related to IkappaB's degradation profiles during early chemical hepatocarcinogenesis
%A Garc¨ªa-Rom¨¢n Rebeca
%A P¨¦rez-Carre¨®n Julio
%A M¨¢rquez-Qui£¿ones Adriana
%A Salcido-Neyoy Martha
%J Journal of Carcinogenesis
%D 2007
%I Medknow Publications
%R 10.1186/1477-3163-6-5
%X Background To define the NF-kappaB activation in early stages of hepatocarcinogenesis and its IkappaB's degradation profiles in comparison to sole liver regeneration. Methods Western-blot and EMSA analyses were performed for the NF-kappaB activation. The transcriptional activity of NF-kappaB was determined by RT-PCR of the IkappaB-¦Á mRNA. The IkappaB's degradation proteins were determined by Western-blot assay. Results We demonstrated the persistent activation of NF-kappaB during early stages of hepatocarcinogenesis, which reached maximal level 30 min after partial hepatectomy. The DNA binding and transcriptional activity of NF-kappaB, were sustained during early steps of hepatocarcinogenesis in comparison to only partial hepatectomy, which displayed a transitory NF-kappaB activation. In early stages of hepatocarconogenesis, the IkappaB-¦Á degradation turned out to be acute and transitory, but the low levels of IkappaB-¦Â persisted even 15 days after partial hepatectomy. Interestingly, IkappaB-¦Â degradation is not induced after sole partial hepatectomy. Conclusion We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-¦Â and IkappaB-¦Á degradation, mainly IkappaB-¦Â degradation, which contributes to gene transcription related to proliferation required for neoplasic progression.
%U http://www.carcinogenesis.com/content/6/1/5