%0 Journal Article %T Changes of Protective Enzyme Activities in Male Sterile Line 160S During Fertility Transformation %A SHEN Liang-yu %A LI Rong-chong %A WANG Rui-xue %A ZOU Yan %J Journal of Chongqing Normal University %D 2012 %I Chongqing Normal University %X "This research studied the activities of the protective enzyme in the leaves and anthers during the fertility transformation of the thermo-sensitive male-sterile line 160S of Brassica napus and selected the fertile rape as the contrast. The protective enzyme activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate-peroxidase (APX) and malondialdehyde (MDA) con-tent were measured. The results showed that the protective enzyme activities of 160S decreased and MDA content accumulated excessively during the fertility conversion, but the fertile line of 31C was of higher protective enzyme activity at the same temperature of the same period, while MDA content was relatively low and the anthers did not atrophy. Those differences of protective enzyme activity and MDA content accumulation were mainly occurred in the anthers of male sterile line 160S under natural temperature conditions. The results showed that one important factor of the fertility transformation of 160S probably was that anti-oxidation enzyme activities were abnormal which result in that the eliminating ability of reactive oxygen species decreased, and the reactive oxygen species and MDA accumulated excessively which probably destroyed the cell membrane. As a result, the pollens developed abnormally, then could not develop the pollen mother cell and behaved abortion traits eventually." %K thermo-sensitive male sterility %K Brassica napus %K anti-oxidation enzyme %K MDA %U http://journal.cqnu.edu.cn/1201/pdf/0115.pdf