%0 Journal Article
%T Production and Purification of Anti-Rhombomys Opimus Immunoglobulins
%A AA Akhavan
%A R Ghods
%A M Jeddi-Tehrani
%A MR Yaghoobi-Ershadi
%J Iranian Journal of Arthropod-Borne Diseases
%D 2011
%I 
%X Background: Zoonotic cutaneous leishmaniasis (ZCL) is an increasing public health problem in some endemic re-gions. Horseradish peroxidase (HRP) conjugated rabbit anti-Rhombomys opimus (R. opimus) Ig is needed for im-munoblotting and ELISA tests used to explore the immune response of the rodents against the sand fly saliva. In this study, the production of HRP conjugated rabbit anti-R. opimus Ig was conducted for the first time.Methods: Rhombomys opimus Ig was purified from serum by protein G affinity chromatography column and in-jected into rabbit to produce anti-R. opimus Ig antibody. The titration of antibody against R. opimus Ig in rabbit se-rum was checked using indirect ELISA. Rabbit anti-R. opimus Ig was purified by Sepharose-4B-R. opimus Ig column. Re-activity of this antibody was assessed by indirect ELISA and was conjugated to HRP by periodate method.Results: Approximately 3.5 mg Ig was purified from 1 ml R. opimus serum using protein G affinity chromatography col-umn. The molecular weight of purified R. opimus Ig was estimated about 150 kDa by SDS-PAGE. Nearly 2.3 mg rab-bit anti-R. opimus Ig was purified from 1 ml immunized rabbit serum. The purified antibody was conjugated to HRP and the optimum titer of HRP conjugated rabbit anti-R. opimus Ig was determined as 1:8000 using direct ELISA.Conclusion: HRP conjugated rabbit anti-Gerbil IgG has been produced by a few companies, but to our knowledge HRP conjugated rabbit anti-R. opimus Ig is not commercially available. Production of HRP conjugated rabbit anti-R. opimus Ig is considerably helpful for immunological studies of R. opimus, the main reservoir host of ZCL in Iran as well as some other countries.
%K Rhombomys Opimus
%K Polyclonal Antibody
%K Zoonotic Cutaneous Leishmaniasis
%K Immunoglobulin
%K Iran
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/19940.pdf&manuscript_id=19940