%0 Journal Article
%T Effect of Shark Liver Oil on Peritoneal Murine Macrophages in Responses to Killed-Candida albicans
%A Monire Hajimoradi
%A Saeed DaneshmandiZuhair Mohammad Hassan
%A 2Maryam Roudbary
%J Iranian Journal of Basic Medical Sciences
%D 2009
%I Mashhad University of Medical Sciences
%X Objective(s)Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency.Materials and MethodsPeritoneal macrophages were separated and cultured (3กม105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. ResultsKilled-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida.ConclusionSimultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement.
%K Candida albicans
%K Macrophage
%K Shark
%K Liver Oi
%U http://www.mums.ac.ir/shares/basic_medical/basicmedjou/88/autumn/hajimorad.pdf