%0 Journal Article
%T Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum
%A Nesrine Azouna
%A Faouzi Jenhani
%A Zohra Regaya
%A Lamia Berraeis
%A Tarek Othman
%A Elfi Ducrocq
%A Jorge Domenech
%J Stem Cell Research & Therapy
%D 2012
%I BioMed Central
%R 10.1186/scrt97
%X In the present study, we compared the effect of various media combining autologous HPL with or without FBS on phenotypic, proliferative and functional (differentiation, cytokine secretion profile) characteristics of human BM-derived MSCs.Despite less expression of adipogenic and osteogenic markers, MSCs cultured in HPL-supplemented media fully differentiated along osteoblastic, adipogenic, chondrogenic and vascular smooth muscle lineages. The analyses of particular specific proteins expressed during osteogenic differentiation (calcium-sensing receptor (CaSR) and parathormone receptor (PTHR)) showed their decrease at D0 before any induction for MSC cultured with HPL mostly at high percentage (10%HPL). The cytokine dosage showed a clear increase of proliferation capacity and interleukin (IL)-6 and IL-8 secretion.This study shows that MSCs can be expanded in media supplemented with HPL that can totally replace FBS. HPL-supplemented media not only preserves their phenotype as well as their differentiation capacity, but also shortens culture time by increasing their growth rate.Mesenchymal stem cells (MSCs) represent a rare population of multipotent progenitors, initially described in bone marrow (BM), giving rise to adipocytes, osteoblasts, chondrocytes, and vascular smooth muscle (VSM)-like hematopoietic supportive stromal cells [1,2]. These cells are capable of multilineage differentiation from a single cell [3,4] and in vivo functional reconstitution of injured tissues in preclinical [5] as well in clinical [6] settings. Thereafter, MSCs have been described in virtually all post-natal organs or tissues [7] but also in fetal adnexa, including both umbilical cord blood [8,9] and placenta [7,10,11]. Today, BM remains the principal source of MSCs in studies investigating their potential use in cell therapy, in spite of significant declines in cell proliferation and differentiation capacity with advancing age [12].MSCs display several common surface antigens, including CD
%U http://stemcellres.com/content/3/1/6