%0 Journal Article
%T Genetic diversity of 28 wild species of fodder legume Cassia using RAPD, ISSR and SSR markers: a novel breeding strategy
%A Suprava Mohanty
%A A. B. Das
%A Nabarun Ghosh
%A B. B. Panda
%J Journal of Biotech Research
%D 2010
%I 
%X RAPD, ISSR and SSR primers were used to assess genetic diversity and phylogenetic relationships among 28 species of Cassia (2n = 16, 26, 28). RAPD, ISSR and SSR primers revealed 36.12, 42.7 and 54.4% polymorphism, respectively. The Dendograms based on RAPD, ISSR, and SSR data precisely organized 28 species of Cassia into different clusters. SSR primer could distinguish all species analyzed within the genus. Polymorphic index varied from 0.1 to 0.5 for both SSR and RAPD markers; primer index values were substantially higher for RAPD primers (0.35 ¨C 4.65) than for SSR primers (0.35 ¨C 1.73). It was possible to identify accessions with the help of RAPD, ISSR and SSR markers. Dendograms constructed from RAPD, ISSR and SSR data revealed DNA marker-based genetic identification in Cassia. Four groups of Cassia that were resolved corresponded to species grouped earlier taxonomically. Cassia mimosoides with a different genomic set up showed close relation to C. javanica. Cassia siamea and C. spectabilis; C. grandis and C. nodosa in a core group with close morphological similarities. Cassia artemisioides and C. covesii, both showing morphologically drought tolerance characters are closely placed, indicating that they are the wild progenitors of these species. The broad adaptation of some species of Cassia implies a large probability that nuclear DNA mutations occurred in the past and that resulted in diversification and genetic polymorphism in growth habit, vigor, and stem, leaf, seed, and pod characteristics and a physiological polymorphism, the water-use efficiency.
%K Cassia
%K genetic markers
%K DNA polymorphism
%K molecular taxonomy
%K plant systematic.
%U http://btsjournals.com/Documents/2010v2p44-55.pdf