%0 Journal Article
%T Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules
%A Hiromitsu Teruya
%A Mariko Tomita
%A Masachika Senba
%A Chie Ishikawa
%A Maki Tamayose
%A Akiko Miyazato
%A Satomi Yara
%A Yuetsu Tanaka
%A Yoichiro Iwakura
%A Jiro Fujita
%A Naoki Mori
%J Retrovirology
%D 2008
%I BioMed Central
%R 10.1186/1742-4690-5-86
%X We tested infection of lung epithelial cells by HTLV-I by coculture studies in which A549 alveolar and NCI-H292 tracheal epithelial cell lines were cocultured with MT-2, an HTLV-I-infected T-cell line. Changes in the expression of several cellular genes were assessed by reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry. Coculture with MT-2 cells resulted in infection of lung epithelial cells as confirmed by detection of proviral DNA, HTLV-I Tax expression and HTLV-I p19 in the latter cells. Infection was associated with induction of mRNA expression of various cytokines, chemokines and cell adhesion molecule. NF-¦ÊB and AP-1 were also activated in HTLV-I-infected lung epithelial cells. In vivo studies showed Tax protein in lung epithelial cells of mice bearing Tax and patients with HTLV-I-related pulmonary diseases.Our results suggest that HTLV-I infects lung epithelial cells, with subsequent production of cytokines, chemokines and cell adhesion molecules through induction of NF-¦ÊB and AP-1. These changes can contribute to the clinical features of HTLV-I-related pulmonary diseases.Human T-cell leukemia virus type I (HTLV-I) is a retrovirus responsible for adult T-cell leukemia (ATL) [1] and a chronic neurological disorder known as HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP) [2,3]. HTLV-I is also implicated in several other inflammatory disorders, such as uveitis, chronic arthropathy and Sj£¿gren's syndrome [4]. Furthermore, transgenic mice expressing Tax protein, a transactivator encoded by HTLV-I, develop proliferative synovitis [5] and exocrinopathies affecting lacrimal and salivary glands, features similar to those of Sj£¿gren's syndrome in humans [6]. Individuals infected with HTLV-I are also known to show pulmonary involvement. For example, patients with HAM/TSP and uveitis or asymptomatic carriers frequently exhibit pulmonary complications characterized by T-lymphocyte alveolitis or lympho
%U http://www.retrovirology.com/content/5/1/86