%0 Journal Article
%T Effects of Polygonum Aviculare Herbal Extract on Proliferation and Apoptotic Gene Expression of MCF-7
%A M. Habibi Roudkenar
%A A. Mohammadi Roushandeh
%A A. Delazar
%A R. Halabian
%J DARU : Journal of Pharmaceutical Sciences
%D 2011
%I BioMed Central
%X Background and the purpose of the study: One of the most common malignancies in women is breast cancer. Although several treatments for breast cancer are available, application of herbal medicine as a supplementary treatment is a new option to help curing the disease. In this study anticancer effects of Polygonum avicular herbal extract was investigated.Methods : Polygonum avicular extract was obtained by methanol. MCF-7 cell line was treated with different concentrations of Polygonum avicular (50, 100, 150, 200, 250, 300,350 400 ng/¦Ěl) for different time lengths ( 6, 12, 24, and 48 hrs). MTT assay and Flow Cytometry were used to evaluate cell proliferation and apoptosis, respectively. RT-PCR was also carried out to evaluate the expression of apoptotic genes. Results and Discussion: Results showed that Polygonum avicular induced cytotoxicity in MCF- 7 cell line at concentrations higher than 300 ng/¦Ěl and this was confirmed by the highest rate of cell death as measured by Trypan Blue and MTT assays. RT-PCR results showed up-regulation of P53 and down-regulation of Bcl-2 proteins which implied the ability of Polygonum avicular to induce apoptosis in MCF-7 cells and confirmed its anticancer property. Further studies are required to evaluate effects of the extract on other apoptotic genes.
%K Herbal Medicine
%K Breast Cancer
%K Knotweed
%K Cell Death
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/19836.pdf&manuscript_id=19836