%0 Journal Article
%T Solvent-Detergent Treatment of IgM-Enriched Immunoglobulin
%A Mojgan Pourmokhtar
%A Rassoul Dinarvand
%A Kamran Mousavi Hosseini
%A Houri Rezvan
%J DARU : Journal of Pharmaceutical Sciences
%D 2003
%I BioMed Central
%X Viral safety of human plasma products plays a key role in their safe uses. Solvent- detergent (SD) virus-inactivation method has gained widespread popularity in the manufacture of biological products. This treatment which inactivates lipid-enveloped viruses effectively consists of incubation of a plasma protein solution in the presence of a non-volatile organic solvent and a detergent. In this study, IgM-enriched immunoglobulin was incubated at 24 ˇăC for 6 h under slow stirring in the presence of tri(n-butyl) phosphate (0.3% w/w ) as solvent and tween 80 (1% w/w) as detergent. After completion of the inactivation process and removal of the solvent-detergent, the ability of SD-treatment to remove Infectious Bovine Rhinotracheitis (IBR) virus (a lipid-enveloped virus) and Foot-and-Mouth Disease virus (a non-enveloped virus) were evaluated by "virus spiking studies" using a scaled down process. Reduction factor of 4 log was obtained for the SD-treatment of IgM-enriched immunoglobulin spiked with IBR virus. No virus inactivation was observed in the SD-treated IgM-enriched immunoglobulin, spiked with Foot-and-Mouth Disease virus. It was concluded that treatment of IgM-enriched immunoglobulin with TNBP-TWEEN 80 may be considered as an efficient lipid-enveloped virus inactivation step in the manufacture of this product.
%K Solvent-detergent
%K Tween 80
%K Bovine rhinotracheitis virus
%U http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/94.pdf&manuscript_id=94