%0 Journal Article
%T Generation of Free Radicals and Enhancement of Hemin-induced Membrane Damage by a Catechol Iron Chelator in Plasmodium falciparum
%A B. Pradines
%A F. Ramiandrasoa
%A T. Fusai
%A A. Hammadi
%J Journal of Biological Sciences
%D 2005
%I Asian Network for Scientific Information
%X We investigated whether FR160, a catechol iron chelator, affects the redox catalytic activity of heme towards membrane lipid, a more physiologically relevant oxidizable substrate. Formation of malondialdehyde (MDA), a major final product of lipid peroxidation initiated by reactive oxygen species, is used as indicator of membrane lipid oxidation. FR160 enhances the ability of hemin to generate MDA by 56%. Vitamin E, an oxygen-radical scavenger, decreases by 30% the formation of MDA by hemin in combination with FR160. When incubated in the presence of hemin, FR160 or artemisinin, alone, react with the amine groups of ¦Â-alanylhistidine (¦Â-AH) and decreases the NBDF-reactive amines of ¦Â-AH. At 48 h of incubation in the presence of hemin, FR160 induces considerable decrease of NBDF-reactive amine of ¦Â-AH by 50%. The iron chelator FR160 acts by generation of radical species and enhance of hemin-catalysed oxidation of membrane lipids. FR160 neither affects the chemical heme crystallization activity nor the production of hemozoin in P. falciparum parasites.
%K Malaria
%K iron chelator
%K siderophore
%K free radical
%K lipid peroxidation
%K antimalarial drug
%U http://docsdrive.com/pdfs/ansinet/jbs/2005/463-471.pdf