%0 Journal Article
%T 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
%A Leguia Mariana
%A Brophy Jennifer AN
%A Densmore Douglas
%A Asante Angel
%J Journal of Biological Engineering
%D 2013
%I BioMed Central
%R 10.1186/1754-1611-7-2
%X There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.
%K 2ab reaction
%K Automated assembly
%K DNA fabrication
%K Synthetic biology
%U http://www.jbioleng.org/content/7/1/2