%0 Journal Article
%T The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts
%A Hesselman Matthijn C
%A Koehorst Jasper J
%A Slijkhuis Thijs
%A Odoni Dorett I
%J Journal of Biological Engineering
%D 2012
%I BioMed Central
%R 10.1186/1754-1611-6-14
%X Synthetic biology is an emerging field that combines molecular biology with engineering principles, which requires abstraction levels applied to a modular biological componentry. The Registry of Standard Biological Parts harbours such a repository of standardized parts, and thereby facilitates the combination of complex molecular modules to novel genetic circuits and devices. However, since finding the best parts for a pre-determined genetic design can be time consuming, we devised the Constructor, a web tool that recommends the smallest number of cloning steps for pre-designed circuits, and implements user-defined quality checks. We present the Constructor ( http://www.systemsbiology.nl/the_constructor) as a constructive web tool that simplifies the in silico assembly of pre-designed gene circuitries from standard parts, reducing both planning and subsequent cloning time.
%K Synthetic Biology
%K BioBrick parts
%K Transcription units
%K Genetic circuit
%U http://www.jbioleng.org/content/6/1/14