%0 Journal Article
%T RESPONSE SURFACE METHODOLOGY FOR THE OPTIMIZATION OF INULINASE PRODUCTION BY K. MARXIANUS VAR. MARXIANUS
%A M.DILIPKUMAR
%A M.RAJASIMMAN
%A N.RAJAMOHAN
%J Journal of Applied Sciences in Environmental Sanitation
%D 2011
%I 
%X Plackett¨CBurman design was employed for screening 18 nutrient components for the production of inulinase using garlic as substrate by Kluyveromyces marxianus var. marxianus in solid-state fermentation (SSF). From the experiments, 4 nutrients viz. Yeast extract, MnSO4.7H2O, K2HPO4 and Corn steep liquor were found to be most significant nutrient components. Hence these 4 components are selected. The selected components were optimized using Response Surface Methodology (RSM). The optimum conditions are: Yeast extract ¨C0.00289 g(gds)-1, MnSO4.7H2O ¨C0.03343 g(gds)-1, K2HPO4 ¨C0.00427 g(gds)-1 and Corn steep liquor -0.05752 g(gds)-1. Under these conditions the production of inulinase was found to be of 463 U(gds)-1.
%K Garlic
%K Inulinase
%K K. marxianus var. marxianus
%K RSM
%K Solid-state fermentation
%U http://trisanita.org/jases/asespaper2011/ases10v6n1y2011.pdf