%0 Journal Article
%T HETEROLOGOUS EXPRESSION OF A CHITINASE GENE FROM AEROMONAS CAVIAEIN PSEUDOMONAS FLUORESCENS
%A AMARILA MAHK
%A ANTONIUS SUWANTO
%A BUDI TJAHJONO
%A BUDI TJAHJONO
%J BIOTROPIA : the Southeast Asian Journal of Tropical Biology
%D 2003
%I SEAMEO, BIOTROP
%X A transcriptional fusion for an Aeromonas caviae chitinase gene was constructed under the control of a constitutive promoter of the kanaraycin resistance gene (PKmR). The construct was inserted into a medium copy number broad host range plasmid vector to yield recombinant plasmid pAM340, which harbored transcriptional fusion PKmR- chi. Another transcriptional fusion, Ptac-chi, in a recombinant plasmid pAM630, was conducted as comparison. Triparental mating of E. coli carrying the recombinant plasmids with Pseudomotws fluorescens 5100, a phyllosphere bacterium, was performed. Pseudomonas fluorescens 5100 exconjugants were examined for constitutive expression of chitinase employing a spectrophotometric assay; they showed stronger chitin degradation activity than Escherichia coli transformants. Using a fungal antagonism plate assay, this chitinolytic P. fluorescens, however, could not inhibit selected phytopathogenic fungi.
%K Aeromonas caviae/ chitinase gene/transcriptional fusion/PKm'V Vtac-chilPseudomonas fluorescens
%U http://journal.biotrop.org/index.php/biotropia/article/view/182/151