%0 Journal Article
%T ASSESMENT OF CYTOTOXIC EFFECT OF METHANOLIC CRUDE EXTRACTS OF AZADIRACTA INDICA
%A Md. Mahabubul Alam Rakib and Mohammad Musarraf Hussain*
%J International Journal of Pharmaceutical Sciences and Research
%D 2013
%I Society of Pharmaceutical Sciences and Research
%X ABSTRACT: The aim of the research is to observe the cytotoxic activity of methanolic crude extracts of Azadirachta indica, a plant belonging to the family Meliaceae. The root of Azadirachta indica was extracted with organic solvent and the extracts were fractionated by using three fractions (n-hexane, ethyl acetate and chloroform). The crude extracts were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent, vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay it can be well predicted that n-hexane, ethyl acetate, chloroform soluble fractions of Azadirachta indica possess cytotoxic principles (LC50 1.429 mg/mL, 2.368 mg/mL and 1.479 mg/mL respectively) comparison with positive control vincristine sulphate (LC50 0.563 mg/mL).
%K Azadirachta indica
%K Meliaceae
%K Methanolic Crude Extracts
%K Cytotoxic Activity
%U http://www.ijpsr.com/V4I4/51%20Vol.%204,%20Issue%204,%20IJPSR,%20April%202013,%20RA-%202189,%20Paper%2051.pdf