%0 Journal Article
%T Production of Homogeneous and Viable Verticillium dahliae Microsclerotia Effective for Verticillium Wilt Studies
%A F.J. Lopez-Escudero
%A C. Mwanza
%A M.A. Blanco-Lopez
%J Biotechnology
%D 2006
%I Asian Network for Scientific Information
%X Artificial production of Verticillium dahliae microsclerotia using potato dextrose agar plates covered by a cellophane sheet was optimized regarding thickness and sterilization method of the film. The objective was to standardize production per plate, homogeneity (size and propagules individuality) and viability (germinability and infective capability) of inoculum, for epidemiological studies. Autoclaved cellophane film, 600 ¦Ìm in thickness, yielded the highest production of microsclerotia, after 7-10 days of incubation at 22¡ãC, without causing its degradation. Amount of microsclerotia per plate was significantly higher for the defoliating (331.9x103) than the non-defoliating (202.1x103) isolate of the pathogen, maintaining the 93.0 and 89.6% of germinability, respectively, after 1 year. Disease developed on cotton plants of cultivars Tabladilla-16 and Tauro, grown on infested soils with 0.5 to 125 microsclerotia per gram of soil, demonstrated its capability of survival and infection. A high relationship between inoculum density and onset and severity symptoms was observed, that depended on the isolate virulence and cultivar susceptibility.
%K Cellophane film
%K cotton
%K defoliating and non-defoliating isolates
%K inoculum
%K inoculum potential
%U http://docsdrive.com/pdfs/ansinet/biotech/2006/421-428.pdf