%0 Journal Article
%T ISOLATION AND CHARACTERIZATION OF MANNANOLYTIC THERMOPHILIC BACTERIA FROM PALM OIL SHELL AND THEIR MANNANASE ENZYME PRODUCTION PROPERTIES
%A SUMARDI
%A ANTONIUS SUWANTO
%A MAGGY T HENAWIDJAJA
%A T RESNAWATI P URWADARIA
%J BIOTROPIA : the Southeast Asian Journal of Tropical Biology
%D 2005
%I SEAMEO, BIOTROP
%X A mannanolytic thermophilic bacterium (L-07) was isolated from palm oil shell after 2 days of enrichment in liquid medium supplemented with 1% palm kernel meal as mannan source. Sequence analysis of 16S-rRNA indicated that L-07 was similar (98%) to Geobacillus stearothermophilus, a species of thermophilic aerobi c bacteria. We found that G. stearothermophilus L-07 produced extracellular ¦Â -1,4-mannanases, but no ¦Â -manosidase and ¦Á -galactosidase activities. The growth of L-07 reached its maximum (3.0 x 106 cell/ml) at 12-20 hours, while the highest ¦Â -mannanase activity (0.52 U/ml) was observed in culture medium after 36 hours of cultivation at 60oC. The medium containing locust bean gum was the best for producing extracellular ¦Â -1,4-mannanases compared with kolang kaling , konjak , and palm kernel meal. SDS-PAGE and zymogram analysis demonstrated that crude mannanase complex of L-07 from locust bean gum containing medium comprised three active bands with molecular weight of 85, 73 and 50 kDa.
%K Extracellular enzyme/mannanase/ Geobacillus stearothermophilus
%U http://journal.biotrop.org/index.php/biotropia/article/view/211/180