%0 Journal Article
%T Validated HPTLC Fingerprint Analysis for Simultaneous Estimation of Quercetin, Kaempferol and Asiatic Acid in Leaves of Two Different Chemotypes of Centella asiatica
%A C Joshi
%A J Savai
%A A Varghese
%A N Pandita
%J International Journal of Pharmaceutical and Biological Research
%D 2012
%I 
%X Quercetin, Kaempferol and Asiatic acid in the methanolic extract of leaves of Two Chemotypes of Centella asiatica was developed and validated. Methods: The HPTLC method was developed using a suitable mobile phase Toluene: Ethyl acetate: Chloroform: Formic acid (6:6:4:1). The plates were derivatized with anisaldehyde reagent and densitometric determination was performed at wavelength 530nm.Results: The Rf values for Asiatic acid, Quercetin, and Kaempferol was 0.33, 0.44 and 0.55 respectively. The linear regression analysis for the calibration showed a linear relationship with r2= 0.9764 and 0.9823 for Quercetin and Kaempferol respectively in the concentration range of 100-1000ng/band. In case of Asiatic acid, the linear regression analysis data for the calibration plots showed a linear relationship with r2=0.9996 in the concentration range of 100-500ng/band. Precision was confirmed by performing replicate analysis on a single day and different days. The accuracy of the method was validated by conducting recovery studies using thestandard addition method. The average recovery for Quercetin was 98.60% for Kaempferol was 90.08% and for Asiatic acid was 97.66%. System suitability parameter was evaluated by spotting five replicates of all three standards. The %RSD was found to be below 2% with respect to peak area as well as Rf value. Robustness ofmethod was evaluated by changing mobile phase concentration from Toluene: Ethyl Acetate: Chloroform: Formic Acid (6:6:4:1) to (5:5:5:1), for which %RSD of peak areas was found to be below 10%.Conclusion: The HPTLC method developed was found to be simple, precise, accurate, suitable and robust for routine quality control of the powder of leaves of Centella asiatica.
%K Centella asiatica
%K Chemotypes
%K HPTLC
%K Linear
%K Precision
%K Robust.
%U http://www.kejapub.com/ijpbr/docs/IJPBR12-03-05-005.pdf