%0 Journal Article
%T In vitro Direct Shoot Organogenesis and Regeneration of Plantlets from Leaf Explants of Sentang (Azadirachta excelsa)
%A Chin Chiew Foan
%A Rofina Yasmin Othman
%J Biotechnology
%D 2006
%I Asian Network for Scientific Information
%X A micropropagation protocol for the production of Azadirachta excelsa plantlets was developed through the formation of shoots using leaf cuttings. Leaf cuttings cultured on Murashige and Skoog (MS) medium supplemented with 2 mg L-1 benzylaminopurine (BAP), .2 mg L-1 kinetin and 6 mg L-1 adenine sulphate produced an average of 5 shoot primordias per leaf cutting. Twenty five percent of these shoot primordias formed shoots with true leaves when cultured on MS medium supplemented with 1 mg L-1 BAP and 12.5 mg L-1 magnesium sulphate. One hundred percent of these shoots formed roots when transferred onto medium supplemented with 10 mg L-1 ¦Á-naphthaleneacetic acid (NAA).
%K Azadirachta excelsa micropropagation
%K leaf cutting
%K shoots primordia
%K shoot organogenesis
%U http://docsdrive.com/pdfs/ansinet/biotech/2006/337-340.pdf