%0 Journal Article
%T Optimization of Electrotransformation Conditions to Improve Genetic Engineering Potential of Lactobacillus spp. Isolated from Gastrointestinal Tract of Chickens
%A C.C. Sieo
%A N. Abdullah
%A W.S. Tan
%A Y.W. Ho
%J Biotechnology
%D 2006
%I Asian Network for Scientific Information
%X The electrotransfromation conditions of Lb. crispatus I12, Lb. brevis I23, Lb. fermentum I25 and Lb. acidophilus I16C, which were isolated from the gastrointestinal tract of chickens, were studied. A series of experiments that involved manipulation of growth and recovery media, cell wall weakening agent, cell concentration, concentration of transforming plasmid, washing buffer and field strength were carried out. The effects of these parameters were found to vary among the Lactobacillus spp. However, under optimal conditions, the transformation efficiencies were improved from 5.4-66.7 transformants/¦Ìg DNA to 102-103 transformants/¦Ìg DNA. The Lactobacillus spp. were also amenable to transformation by various plasmids such as pGK12, pLP3537, pLP825 and pSA3. This indicated that these strains could be potential candidates for bioengineering to improve feed degradation and growth performance of chickens.
%K Chicken
%K electroporation
%K Lactobacillus
%K optimization
%K transformation
%U http://docsdrive.com/pdfs/ansinet/biotech/2006/244-251.pdf