%0 Journal Article
%T Fast and Sensitive Interferon-¦Ã Assay Using Supercritical Angle Fluorescence
%A Christian M. Winterflood
%A Thomas Ruckstuhl
%A Stefan Seeger
%J Biosensors
%D 2013
%I MDPI AG
%R 10.3390/bios3010108
%X We present an immunoassay for Interferon-¦Ã (IFN-¦Ã) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
%K one-step immunoassay
%K interferon-gamma
%K supercritical angle fluorescence
%K polymer test tube
%U http://www.mdpi.com/2079-6374/3/1/108