%0 Journal Article
%T Validated HPLC fingerprint analysis for Simultaneous Determination of Quercetin and Kaempferol in Methanolic Extract of Tridax procumbens
%A R. Shah
%A N. Sharma
%A V. Patel
%A J. Savai
%J International Journal of Pharmaceutical and Biological Research
%D 2012
%I 
%X Aim: A rapid, sensitive and accurate Reversed Phase-High Performance Liquid Chromatography method (RPHPLC)with Photo-diode array Detection (PDA) was developed and validated for the simultaneous determination of Quercetin and Kaempferol in the methanolic extract of Tridax procumbens plant. Method: Whole plant of Tridax procumbens was collected from Dhule District, Shirpur; dried and methanolic extract was prepared and analyzed for the presence of standards - Quercetin and Kaempferol using TLC and RPHPLC method and detected at ¦Ë=240nm.Results: The Rf values for Quercetin, and Kaempferol was 0.35 and 0.48 respectively. There was a linear relationship between chromatographic area and concentration of analytes over the concentration range 1-40 ¦Ìg/ml for both, Quercetin and Kaempferol with the retention time of about 8.9 minutes and 10.6 minutesrespectively and correlation coefficient of linear regression analysis was found to be r2= 0.999 and detection limits were between 0.164-0.289¦Ìg/ml for Quercetin and 0.497-0.816 ¦Ìg/ml for Kaempferol. The average percentage recovery was found to be 98.02% and 98.80% for Quercetin and Kaempferol respectively. Conclusion: The RP-HPLC method developed was found to be simple, sensitive, precise and accurate forroutine quality control of the powder of whole plant of Tridax procumbens.
%K Linear
%K Recovery
%K Simple
%K Sensitive
%K precise.
%U http://www.kejapub.com/ijpbr/docs/IJPBR12-03-04-10.pdf