%0 Journal Article
%T Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers
%A Shradha Prabhulkar
%A Adam de la Zerda
%A Amit Paranjape
%A Richard M. Awdeh
%J Biosensors
%D 2013
%I MDPI AG
%R 10.3390/bios3010077
%X A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool.
%K gold nanorods
%K optical coherence tomography
%K protein biomarkers
%K immunoassay
%K surface plasmon resonance
%K glucose transporter-17£¿
%U http://www.mdpi.com/2079-6374/3/1/77