%0 Journal Article
%T In - vitro Antioxidant Potential from the Leaves of Punica granatum Linn. Grown in Bangladesh
%A Hemayet Hossain
%A Tanzir Ahmed
%A Md. Sariful Islam Howlader
%A Rajan Sen
%J International Journal of Pharmaceutical and Phytopharmacological Research
%D 2013
%I International Journal of Pharmaceutical and Phytopharmacological Research
%X The crude ethanolic extract of the leaves of Punica granatum Linn.(Family:Punicaceae)was evaluated for its possibleantioxidant activities growing in northeast part of Bangladesh. Five complementary test systems, namely DPPH free radicalscavenging, nitric oxide scavenging, reducing power, Fe++ion chelating ability and total phenolic content were used fordetermining antioxidant activities of the leaf extract. In DPPH free radical scavenging test, IC50value for ethanolic crude extractwas found fairly significant23.45¡À0.062 ¦Ìg/ml while compared to the IC50value of the reference standards ascorbic acid andButylated Hydroxy Anisole (BHA) (5.23¡À0.034 and6.15¡À0.027 ¦Ìg/ml) respectively. The ethanol extract showed maximum nitricoxide (NO) scavenging activity of 76.55¡À0.89% at 100 ¦Ìg/ml, where as ascorbic acid exhibited 88.43 ¡À 0.81% inhibition atthe same concentration. The IC50value for nitric oxide scavenging test wasalso found significant (55.65¡À0.072 ¦Ìg/ml) whilecompared to the IC50value of the reference standard ascorbic acid (31.38¡À0.047 ¦Ìg/ml). The maximum absorbance for reducingpower assay was found to2.015at 100 ¦Ìg/ml while compared to2.817and 2.031 for standard ascorbic acid and BHArespectively. TheIC50value of the extract as percentage of Fe++ion chelating ability was determined as65.109¦Ìg/ml where thatof EDTA showed8.75¦Ìg/ml. The total phenolic amount was also calculated as quite high in ethanolic crude extract378.37mg/gof gallic acid equivalent. Therefore, the obtained results tend to suggest the antioxidant activities of the crude ethanolicextract ofthe leaves of P. granatum and justify its use in folkloric remedies.
%K Punica granatum
%K DPPH free - radical sca venging
%K Nitric oxide scavenging
%K Reducing power
%K Total phenolic.
%U http://www.eijppr.com/nov-dec2012/4.pdf