%0 Journal Article
%T PURIFICATION AND CHARACTERIZATION OF LACCASE PRODUCED BY Schyzophylum commune IBL-06 IN SOLID STATE CULTURE OF BANANA STALKS
%A Muhammad Irshad
%A Muhammad Asgher
%A Munir Ahmad Sheikh
%A Haq Nawaz
%J BioResources
%D 2011
%I North Carolina State University
%X Shyzophyllum commune IBL-06 produced 367 IU/mL of laccase in solid state bioprocess of banana stalk under optimum physical and nutritional parameters. The optimum SSF conditions were §âH 4.5; temperature, 35¡ãC; inoculum size, 3mL (106-108 mL-1); moisture content, 60%; C:N ratio in the medium, 15:1 (glucose and ammonium nitrate as additional carbon and nitrogen sources, respectively), ABTS (1mM), 1 mL, and CuSO4 (1mM), 1mL. Laccase was purified 3.95-fold with specific activity of 158 U/mg by ammonium sulfate precipitation, followed by gel filtration chromatography using Sephadex G-100 column. Molecular weight of the laccase was 63 KDa on SDS-PAGE. Purified laccase had an optimum §âH of 6.0 and was stable in the §âH range from 6 to 7. The optimum temperature was 40 ¡ãC, and it displayed considerable stability within the range 30 to 35 ¡ãC with 24 h incubation. Km and Vmax were 0.025 mM and 80 mM/min, respectively, using ABTS as a substrate. Copper sulfate increased the activity of purified laccase when used at low concentration, but silver nitrate exerted the strongest inhibition.
%K Laccase
%K Shyzophylum commune IBL-06
%K Banana stalk
%K Purification
%K Characterization
%U www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2861_Irshad_ASN_Purific_Char_Laccase_SS_Cult_Banana_Stalks_1708.pdf