%0 Journal Article
%T In vitro effect of caffeine on internal mammary artery rings used in cardiac revascularization surgery Acci車n in vitro de la cafe赤na en anillos de arteria mamaria interna utilizada en cirug赤a de revascularizaci車n cardiaca
%A Dar赤o Echeverry
%A F谷lix R. Montes
%A Alexandra Delgadillo
%A Marcela Beltr芍n
%J Biom谷dica
%D 2008
%I Instituto Nacional de Salud
%X Introduction. The vasodilator effect of caffeine in animal models arteries has been demostrated previously. However, studies with the same methodology using human arteries in vitro have not been performed. Objectives. The in vitro vasoactive effects of caffeine was evaluated on human internal mammary arteries. Materials and methods. Internal mammary artery rings were used (n=20). Endothelial function was evaluated with acetylcholine at a concentration of 3.16x10 -6 M, nitroglycerine at cumulative concentrations of 10 每11 M to 10 每4 M and caffeine with cumulative concentrations of 10 每8 M to 10 每4 M. Results. Nitroglycerin produced a maximum relaxation percentage of 87.4㊣12.3%, caffeine a percentage of 86.9㊣21.0% in arteries with functional endothelium, and of 71.6㊣28.6% in arteries with endothelial dysfunction. No differences were detected among the three groups ( p=0.289). Similarly, no differences were found between EC 50 in arteries with functional endothelium (1.66x10 -5 ㊣1.57x10 -5 M) and dysfunctional arteries (7.8 x10 -5 ㊣14.6 x10 -5 M). Nitroglycerine proved more potent than caffeine (EC 50 = 4.3 x10 -9 ㊣4.4x10 -9 M) ( p=0.013). Conclusions. Although nitroglycerin was a more potent vasodilator, caffeine had a strong arterial vasodilator effect regardless of endothelial function in human arteries. Introducci車n. El efecto vasodilatador de la cafe赤na en las arterias de modelos animales ya ha sido demostrado. Se desconocen estudios con la misma metodolog赤a in vitro utilizando arterias humanas. Objetivos. Evaluar los efectos vasoactivos in vitro de la cafe赤na en la arteria mamaria interna de humanos. Materiales y m谷todos. Se utilizaron 80 anillos de arteria mamaria interna (n=20 pacientes). La funcionalidad del endotelio se evalu車 con acetilcolina a una concentraci車n de 3,16x10-6M, de nitroglicerina con dosis acumulativas de 10 -11 M a 10 -4 M y de cafe赤na con concentraciones acumulativas de 10 -8 M a 10-4 M. Resultados. La nitroglicerina indujo un porcentaje m芍ximo de relajaci車n de 87,4㊣12,3%, la cafe赤na, de 86,9㊣21,0% en arterias con endotelio funcional y de 71,6㊣28,6% en arterias con disfunci車n endotelial. No se encontraron diferencias entre los tres grupos ( p=0,289). Tampoco se encontraron diferencias en la EC 50 en arterias con endotelio funcional (1,66x10 -5 ㊣1,57x10 -5 M) y arterias disfuncionales (7,75x10 -5 ㊣14,64x10 -5 M). La nitroglicerina result車 m芍s potente que la cafe赤na (EC 50 = 4,30x10 -9 ㊣4,35x10 -9 M) ( p=0,013). Conclusiones. Aunque la nitroglicerina fue un vasodilatador m芍s potente, la cafe赤na tuvo un fuerte efecto vasodilatador
%K cafe赤na/uso terap谷utico
%K revascularizaci車n mioc芍rdica
%K vasodilataci車n
%K aorta
%K ateroesclerosis
%K endotelio
%K acetilcolina
%U http://www.revistabiomedica.org/index.php/biomedica/article/view/101