%0 Journal Article
%T Isolate of Encephalitozoon intestinalis from stools of a Colombian patient with AIDS Primer aislamiento de Encephalitozoon intestinalis a partir de muestra de materia fecal de un paciente colombiano con sida
%A Ana Luz Galv芍n
%A Katherine Bedoya
%A Martha Nelly Montoya
%A Jorge Botero
%J Biom谷dica
%D 2008
%I Instituto Nacional de Salud
%X Introduction. Microsporidia are obligate intracellular parasites that are recognized as important opportunistic pathogens of immunocompromised and transplanted patients. Enterocytozoon bieneusi and, less frequently, Encephalitozoon intestinalis are the most prevalent species in humans; both of them are associated with enteric infections. Cell cultures have been useful in the study of microsporidia biology. In Colombia, however, no isolates of microsporidia from patients with AIDS have been obtained. Objective. A cell culture of intestinal microsporidia was established from stools of positive patients in order to isolate a native strain. Materials and methods. Stool from a single AIDS patient was concentrated with the water-ether technique, and the sediment was treated with a mixture of antibiotics and antifungal agents for 18 hours at 37o C. Vero cells were cultivated in 24-well plates with Gibco  RPMI medium supplemented with 10% bovine fetal serum and antibiotics. The culture was subsequently inoculated with previously concentrated spores. The medium was changed every second day and the presence of spores was evaluated with the Quick Hot Gram chromotrope stain. Results. Two weeks post-infection, microsporidial spores were identified with characteristic morphology and staining properties. PCR results showed that Encephalitozoon intestinalis was the isolated species. Conclusions. A cell culture of microsporidia was established from a stool sample. This protocol is important to isolate and maintain additional native Colombian strains and it will contribute to biochemical, immunological and epidemiological studies of the currently established strain. Introducci車n. Los microsporidios son agentes de infecciones oportunistas en pacientes con sida y con trasplantes, principalmente. Enterocytozoon bieneusi y Encephalitozoon intestinalis son los m芍s frecuentes, asociados con infecciones ent谷ricas. Los cultivos celulares han contribuido al conocimiento de los microsporidios. En Colombia no se han obtenido aislamientos provenientes de pacientes con microsporidiosis y, por consiguiente, no existen cepas aut車ctonas de los mismos. Objetivo. Establecer el cultivo celular de microsporidios intestinales a partir de materia fecal de pacientes parasitados. Materiales y m谷todos. Se realiz車 concentraci車n agua-谷ter de la materia fecal positiva para microsporidios y el sedimento resultante se trat車 con una mezcla de antibi車ticos y antimic車ticos durante 18 horas a 37 oC. Se inocularon c谷lulas Vero previamente cultivadas en placas de 24 pozos y en medio RPMI con suplemento de
%K Encephalitozoon
%K microsporidiosis
%K s赤ndrome de inmunodeficiencia adquirida
%K aislamiento de pacientes
%K c谷lulas cultivadas
%K heces
%U http://www.revistabiomedica.org/index.php/biomedica/article/view/83