%0 Journal Article
%T Proteome analysis of the hyaluronic acid-producing bacterium, Streptococcus zooepidemicus
%A Esteban Marcellin
%A Christian W Gruber
%A Colin Archer
%A David J Craik
%A Lars K Nielsen
%J Proteome Science
%D 2009
%I BioMed Central
%R 10.1186/1477-5956-7-13
%X Using hyaluronidase to remove the capsule and by optimising cellular lysis, a reference map for S. zooepidemicus was completed. This protocol significantly increased protein recovery, allowing for visualisation of 682 spots and the identification of 86 proteins using mass spectrometry (LC-ESI-MS/MS and MALDI-TOF/TOF); of which 16 were membrane proteins.The data presented constitute the first reference map for S. zooepidemicus and provide new information on the identity and characteristics of the more abundantly expressed proteins.Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a commensal of the upper respiratory tract, skin and urogenital tract of horses, some other animals and humans. It is a primarily opportunistic pathogen in many animal species, including domesticated animals such as horses, cows, pigs, sheep, and dogs. It is the primary cause of equine respiratory tract infections in foals and the leading cause of infertility in mares [1,2]. Sequencing of strain H70 by the Sanger Institute was sponsored by The Horserace Betting Levy Board in UK.S. zooepidemicus also infects humans, typically through zoonotic transmission from domesticated animals or by ingestion of improperly pasteurised dairy products [3]. Clinical manifestations in humans include mild respiratory disease, pneumonia, endocarditis, endophthalmitis, septic arthritis, meningitis, septicemia, streptococcal toxic shock syndrome and post-streptococcal glomerulonephritis. A large outbreak with 253 cases of acute nephritis in Brazil was linked to the consumption of unpasteurised cheese and a strain isolate, MGCS10565, was recently sequenced [4].Lancefield Group A and C streptococci produce the exopolysaccharide hyaluronic acid (HA). The HA capsule protects streptococci from phagocytosis [5-8] and may facilitate tissue adherence and invasion [5,9]. The HA capsule is an important virulence factor for S. pyogenes and disruption of the has operon results in a significant reduction in pathog
%U http://www.proteomesci.com/content/7/1/13