%0 Journal Article
%T A comparison between protein profiles of B cell subpopulations and mantle cell lymphoma cells
%A Henrik Stranneheim
%A Lukas M Orre
%A Janne Lehti£¿
%A Jenny Flygare
%J Proteome Science
%D 2009
%I BioMed Central
%R 10.1186/1477-5956-7-43
%X Subpopulations of B cells representing the germinal centre (GC), the pre-GC mantle zone and the post-GC marginal zone were isolated from tonsils using automated magnetic cell sorting (AutoMACS) of cells based on their expression of CD27 and IgD. Protein profiling of the B cell subsets, of cell lines representing different lymphomas and of primary MCL samples was performed using top-down proteomics profiling by surface-enhanced laser detection/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Quantitative MS data of significant protein peaks (p-value < 0.05) separating the three B-cell subpopulations were generated. Together, hierarchical clustering and principal component analysis (PCA) showed that the primary MCL samples clustered together with the pre- and post-GC subpopulations. Both primary MCL cells and MCL cell lines were clearly separated from the B cells representing the GC compartment.AutoMACS sorting generates sufficient purity to enable a comparison between protein profiles of B cell subpopulations and malignant B lymphocytes applying SELDI-TOF-MS. Further validation with an increased number of patient samples and identification of differentially expressed proteins would enable a search for possible treatment targets that are expressed during the early development of MCL.In the last decade, several studies of lymphomas have been performed using microarray analysis of global gene expression [1]. Genomic profiling has proven to be of both diagnostic and prognostic value. However, the correlation between mRNA and protein expression is generally poor, and regulation of many physiological processes is post-transcriptional. Protein analysis is therefore essential for the elucidation of biological mechanisms behind e.g. lymphoma formation. Proteomic profiling using liquid chromatography-MS/MS has been applied to a limited number of lymphomas of different origin [2]. The high throughput top-down method of SELDI-TOF-MS, which is based on chip-binding of i
%U http://www.proteomesci.com/content/7/1/43