%0 Journal Article
%T Visible Genotype Sensor Array
%A Yuichi Michikawa
%A Tomo Suga
%A Yoshimi Ohtsuka
%A Izumi Matsumoto
%J Sensors
%D 2008
%I MDPI AG
%X A visible sensor array system for simultaneous multiple SNP genotyping has been developed using a new plastic base with specific surface chemistry. Discrimination of SNP alleles is carried out by an allele-specific extension reaction using immobilized oligonucleotide primers. The 3 бщ ?  -ends of oligonucleotide primers are modified with a locked nucleic acid to enhance their efficiency in allelic discrimination. Biotin-dUTPs included in the reaction mixture are selectively incorporated into extending primer sequences and are utilized as tags for alkaline phosphatase-mediated precipitation of colored chemical substrates onto the surface of the plastic base. The visible precipitates allow immediate inspection of typing results by the naked eye and easy recording by a digital camera equipped on a commercial mobile phone. Up to four individuals can be analyzed on a single sensor array and multiple sensor arrays can be handled in a single operation. All of the reactions can be performed within one hour using conventional laboratory instruments. This visible genotype sensor array is suitable for  бщ ?  focused genomics бщ ?   that follows  бщ ?  comprehensive genomics бщ ?  .
%K Visible sensor
%K SNP
%K array
%K plastic
%K primer extension.
%U http://www.mdpi.com/1424-8220/8/4/2722/