%0 Journal Article
%T Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins
%A Lijun Zhang
%A Xiaofang Jia
%A Xiaojun Zhang
%A Jianjun Sun
%A Xia Peng
%A Tangkai Qi
%A Fang Ma
%A Lin Yin
%A Yamin Yao
%A Chao Qiu
%A Hongzhou Lu
%J Proteome Science
%D 2010
%I BioMed Central
%R 10.1186/1477-5956-8-12
%X 26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. Globally, there are estimated 33.4 million living individuals in 2008 according to AIDS epidemic update 2009 by the World Health Organization (WHO) http:/ / www.unaids.org/ en/ KnowledgeCentre/ HIVData/ EpiUpdate/ EpiUpdArchive/ 2009/ default.asp webcite. Current therapies direct against viral proteins. Highly active anti-retroviral therapy has been problematic because of long-term toxicity, inhibitor resistance, and the inability to target persistent reservoirs[1,2]. Thus, there is a need for the comprehensive elucidation of HIV-1-mediated effects on host cellular protein networks and unique protein targets for the design of therapeutic drugs.Over the past few decades, HIV-mediated effects on host cells have typically involved the study of one gene or one protein at a time, to elucidate their functions in lymphocyte or monocyte biology, signaling pathways or immune system [3-5]. However,
%U http://www.proteomesci.com/content/8/1/12