%0 Journal Article
%T Treatment with captopril abrogates the altered expression of alpha1 macroglobulin and alpha1 antiproteinase in sera of spontaneously hypertensive rats
%A Norhaniza Aminudin
%A Nur-Atiqah H Abdullah
%A Hasni Misbah
%A Saiful A Karsani
%A Ruby Husain
%A See Z Hoe
%A Onn H Hashim
%J Proteome Science
%D 2012
%I BioMed Central
%R 10.1186/1477-5956-10-17
%X Five proteins of high abundance were found to be significantly altered when the 2-DE serum profiles of the SHR were compared to those that were similarly generated from the SDR. Analysis by mass spectrometry and database search identified the proteins as retinol binding protein 4, complement C3, albumin (19.9 kDa fragment), alpha1 macroglobulin and alpha1 antiproteinase, which are all known to be associated with hypertension. The altered expression of the two latter proteins was found to be abrogated when similar analysis was performed on sera of the SHR that were treated with captopril.Our data suggests that serum alpha1 macroglobulin and alpha1 antiproteinase are potentially useful complementary biomolecular indicators for monitoring of hypertension.Spontaneously hypertensive rats (SHR) have been widely used as an animal model to investigate primary hypertension and its relationship to cardiovascular diseases. The SHR strain was generated in the 1960s by Okamoto et al. by selective breeding of the Wistar-Kyoto rats with high blood pressure [1]. The blood pressure of SHR usually rises at around 5-6 weeks of age and the systolic pressure of an adult SHR may reach a value of between 180 and 200 mmHg. The SHR usually develops characteristics of cardiovascular diseases like hypertrophy of the heart and blood vessels, which start at around 40 weeks of age [2].Hypertension has been known to cause the altered levels of serum or plasma proteins. A considerable number of proteins have been previously reported to be altered in levels in the sera of both animals and human subjects [3-6]. While some of the proteins were thought to be involved as anti-inflammatory and protective response [4,7], others were related to endothelial vascular repair [5], arterial smooth muscle cell growth [6] and some may instead be the contributing factors of hypertension.In the present study, we have investigated the simultaneous expression of the high abundant serum proteins in the normotensive S
%K hypertension
%K biomarker
%K spontaneously hypertensive rats
%K serum proteins
%K proteomics
%U http://www.proteomesci.com/content/10/1/17