%0 Journal Article
%T Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis
%A Apiradee Hongsthong
%A Matura Sirijuntarut
%A Rayakorn Yutthanasirikul
%A Jittisak Senachak
%A Pavinee Kurdrid
%A Supapon Cheevadhanarak
%A Morakot Tanticharoen
%J Proteome Science
%D 2009
%I BioMed Central
%R 10.1186/1477-5956-7-33
%X High temperature stresses are well known to cause protein aggregation and denaturation, and in order to cope with these stress, a cellular response occurs. Proteomics research regarding cellular responses to high temperature stresses was carried out in bacteria. The majority of the differentially expressed proteins belong to the group of proteins including heat shock responsive chaperones and proteases, of which many are also induced in response to gamma irradiation and/or desiccation [1,2]. In addition to these proteins, some central metabolic proteins were also found by Fourier transform ion cyclotron resonance (FTIR) mass spectrometric proteomics analysis [2]. Thus, it was hypothesized that elevated temperature may induce a general stress response, and could lead to cross-protection against related stresses [2].In cyanobacteria, gene regulation mediated by high temperature stresses has been studied less extensively than regulatory responses to low temperature stresses. In some cyanobacteria, such as Synechocystis, Synechococcus and Nostoc, heat shock responses have been investigated [3-5]. An alternative sigma factor (SigH) and heat-shock protein (HSP) were both significantly induced immediately following exposure to heat stress [6,7].Since Spirulina cells are grown in outdoor ponds for mass cultivation, they are exposed to various stress conditions, including high temperature stress. During daylight hours in tropical countries, the cells are exposed to high temperatures of around 40ˇăC. The temperature fluctuation in outdoor mass cultivation has a serious effect on biomass yield and the biochemical content of the cells. Some components of Spirulina cells have pharmaceutical benefits, such as unsaturated fatty acids. The level of unsaturated fatty acids in membrane lipids has been shown to play a critical role in response to temperature change in various organisms. Substantial evidence points to an association between fatty acid desaturation and temperature stress
%U http://www.proteomesci.com/content/7/1/33