%0 Journal Article
%T A proteomic view of Caenorhabditis elegans caused by short-term hypoxic stress
%A Hualing Li
%A Changhong Ren
%A Jinping Shi
%A Xingyi Hang
%A Feilong Zhang
%A Yan Gao
%A Yonghong Wu
%A Langlai Xu
%A Changsheng Chen
%A Chenggang Zhang
%J Proteome Science
%D 2010
%I BioMed Central
%R 10.1186/1477-5956-8-49
%X Here, we utilized a quantitative proteomic approach to evaluate changes in the expression patterns of proteins during the early response to hypoxia in C. elegans. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to compare the proteomic maps of wild type C. elegans strain N2 under a 4-h hypoxia treatment (0.2% oxygen) and under normoxia (control). A subsequent analysis by MALDI-TOF-TOF-MS revealed nineteen protein spots that were differentially expressed. Nine of the protein spots were significantly upregulated, and ten were downregulated upon hypoxic stress. Three of the upregulated proteins were involved in cytoskeletal function (LEV-11, MLC-1, ACT-4), while another three upregulated (ATP-2, ATP-5, VHA-8) were ATP synthases functionally related to energy metabolism. Four ribosomal proteins (RPL-7, RPL-8, RPL-21, RPS-8) were downregulated, indicating a decrease in the level of protein translation upon hypoxic stress. The overexpression of tropomyosin (LEV-11) was further validated by Western blot. In addition, the mutant strain of lev-11(x12) also showed a hypoxia-sensitive phenotype in subsequent analyses, confirming the proteomic findings.Taken together, our data suggest that altered protein expression, structural protein remodeling, and the reduction of translation might play important roles in the early response to oxygen deprivation in C. elegans, and this information will help broaden our knowledge on the mechanism of hypoxia response.Hypoxic stress can induce apoptosis but also trigger adaptive mechanisms for cell survival. Mammalian cells respond to hypoxia by changes in the expression of numerous genes and proteins to increase anaerobic energy production, protect cells from hypoxic stress, and increase local angiogenesis [1,2]. Recently, the nematode Caenorhabditis elegans (C. elegans) has been proven to be an valuable model organism for studying the molecular response to hypoxia [3,4]. Although C. elegans is sensitive to hypoxic stress, r
%U http://www.proteomesci.com/content/8/1/49